Researchers at the University of Illinois at Urbana-Champaign have developed technology that enables a smartphone to perform lab-grade medical diagnostic tests that typically require large, expensive instruments. Costing only $550, the spectral transmission-reflectance-intensity (TRI)-Analyzer from Bioengineering and Electrical & Computer Engineering Professor Brian Cunningham’s lab attaches to a smartphone and analyses patient blood, urine, or saliva samples as reliably as clinic-based instruments that cost thousands of dollars.
“Our TRI Analyzer is like the Swiss Army knife of biosensing,” said Cunningham, the Donald Biggar Willett Professor of Engineering and director of the Micro + Nanotechnology Lab at Illinois. “It’s capable of performing the three most common types of tests in medical diagnostics, so in practice, thousands of already-developed tests could be adapted to it.”
In a recently published paper, Cunningham’s team used the TRI Analyzer to perform two commercially available assays—a test to detect a biomarker associated with pre-term birth in pregnant women and the PKU test for newborns to indirectly detect an enzyme essential for normal growth and development. Their tests results were comparable to those acquired with clinic-grade spectrometer instrumentation.
“The TRI Analyzer is more of a portable laboratory than a specialized device,” said Kenny Long, an MD/PhD student and lead author of the research study.
Among the many diagnostic tests that can be adapted to their point-of-care smartphone format, Long said, is an enzyme-linked immunosorbent assay (ELISA), which detects and measures a wide variety of proteins and antibodies in blood and is commonly used for a wide range of health diagnostics tests. The system is capable of detecting the output of any test that uses a liquid that changes colour, or a liquid that generates light output (such as from fluorescent dyes).
The TRI Analyzer operates by converting the smartphone camera into a high-performance spectrometer. Specifically, the analyser illuminates a sample fluid with the phone’s internal white LED flash or with an inexpensive external green laser diode. The light from the sample is collected in an optical fibre and guided through a diffraction grating into the phone’s rear-facing internal camera. These optical components are all arranged within a 3D-printed plastic cradle.
The TRI Analyzer can simultaneously measure multiple samples by using a microfluidic cartridge that slides through an opening in the back of the cradle. This ability to analyse multiple samples quickly and reliably makes the Analyzer suitable for patients who lack convenient access to a clinic or hospital with diagnostic test facilities or for patients with urgent health situations requiring rapid results.
University of Illinois at Urbana-Champaign